IUBio

FACS: excluding dead cells?

Hiromi Tagoh htagoh at hotmail.com
Sun Feb 6 06:11:38 EST 2000


The fluorescence wavelength can overlap between PI and PE, so PE's window
can detect PI, but even if so, if you will exclude PI positive cells, NO
PROBLEM!!  And when you set the detection level and  compensation as well as
you can, it is really rare that your machine recognise PE positive cells as
PI positive meaning dead cells and exclude them, and you will miss the PE
bright cells.





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