Have you tried damping the background signal with albumin? If that doesn't
work why not try a modified tunnel assay = ISEL (In Situ-Nick end
labeling). If strepavidin is your amplifyer, what concentration of biotin
are you using if any. You might want to tweek the
concentrations...Immunohistochem is an art form more than it is a
science...we learn the hard way.
"J. E. CASTELLANOS PARRA" wrote:
> I am trying to do an brain immunohistochemistry of MCP1 citokine on
> cryostat brain slides using Cy3 conjugated streptavidin, but it does not
> give well, the slide control without primary antibody is very similar to
> slide treated with antibody. Whath do to do????
> Jaime Castellanos
> Unite NRSN
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