i've used the BD method with success, what exactly is your question?
Andrew Hall wrote:
> Hi all,
>> Im looking for some advice concerning the best way to do intracellular
> cytokine/surface marker staining (three colour). Basically I have tried it a
> few times using FACS perm (BD) to permeabilise the cells (antigen stimulated
> human PBMC) and anti-cytokine/CD marker antibodies (BD) with reasonable
> results. We are planning to expand these experiments and as a result I need
> to buy in the antibodies and check the protocol for this type of staining.
> Although these experiments have been relatively succesful so far I want to
> cover all options before paying out £3000 for all the reagents we will need
> to use.
> The alternatives I have in mind are Dako and Coulter and I am planning to
> get in touch with these companies to see what the have on offer. However any
> advice would be appreciated.
>> Andrew Hall
> Univesity of Aberdeen
