My question was intended to be a question on how to prepare
lymphocytes for FACS. I am a FACS neophyte. Suppose I was interested
in Th1 responses to a particular antigen. I could do a FACS on
interferon gamma and CD4. Since I am mainly interested in humans, I
would be getting lymphocytes from people with a remote history of
infection and relying on their recall responses. So what is the best
way to elicit these responses? - Kevin Ault
Bruce Smith <Bruce.Smith at Mail.TJU.Edu> wrote:
>>Is this a question about cytometry or culture?
>>Kevin Ault wrote:
>>> I have a rather basic question about FACS as well. If one is looking
>> for a response to a certain antigen, what is the best source of
>> antigen presenting cells? Autologous irradiated macrophages? Mixed
>> peripheral mononuclear cell cultures? Something else? I am mainly
>> interested in humans. Please post replies to the group. - Kevin
>> Ault MD University of Iowa (kevin-ault at uiowa.edu)
>
