Alec-
MHC class I molecules on the surface of cultured cells are RESISTANT to
trypsin treatments used to suspend the cells. I do this routinely to
study expression by FACS. Purified MHC class I molecules are sensitive
to relatively large amounts of trypsin. Sorry I cannot cite a reference
off the top of my head, but it has been shown by different groups.
I hope this helps.
-Dave
--
David R. Johnson, Ph.D.
Research Scientist, Department of Pathology
454 BCMM, 295 Congress Avenue
Yale Medical School, New Haven, CT 06510 USA
Tel.: 203/737-2298, Fax: 203/737-2293
http://johnsond3.med.yale.edu
