I have been debating whether to use BFA or monensin to block golgi secretion
of cytokine before intracellular flow cytometery staining of human PBMC
cultures. As far as I am aware monensin is probably the best candidate since
I have read that BFA can affect the expression of some CD markers (CD69)
although it apparently has greater toxicity. However I have been unable to
find a protocol or paper with the correct concentration and volume of
monensin to add to a given number of cells. Any suggestions to these queries
would be appreciated.
--
Andrew Hall
University of Aberdeen
andrew.hall at virgin.net