Hi,
I haven't actually done any intracellular staining for cytokines, however,
it was a hot topic on the Purdue Cytometry Mailing list a while back. I
believe that the consensus was that the choice between monensin and BFA
depended upon the cytokine in question. It would probably be helpful to
check out the mailing list archive at
www.cyto.purdue.edu/hmarchiv/index.htm.
Cheers,
Wallace Lauzon, PhD
Dept. Biochemistry, Microbiology and Immunology
University of Ottawa
451 Smyth Rd
Ottawa, Ontario
CANADA
K1H 8M5
"Andrew Hall" <andrew.hall at virgin.net> wrote in message
news:JaaA4.896$Fy1.15504 at news2-win.server.ntlworld.com...
> I have been debating whether to use BFA or monensin to block golgi
secretion
> of cytokine before intracellular flow cytometery staining of human PBMC
> cultures. As far as I am aware monensin is probably the best candidate
since
> I have read that BFA can affect the expression of some CD markers (CD69)
> although it apparently has greater toxicity. However I have been unable to
> find a protocol or paper with the correct concentration and volume of
> monensin to add to a given number of cells. Any suggestions to these
queries
> would be appreciated.
>> --
> Andrew Hall
> University of Aberdeen
>andrew.hall at virgin.net>>>
