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How to interpreter these results: Sephadex limits 5000 Daltons Vitamin B12 : 1350 Daltons Ve vitamin B12 = 8,7 mL Vt sephadex G-25 = 5,3 mL Vo = 5,7 I don't understand why Ve > Vt, is it possible? -- - Onyryc - onyryc at free.fr http://onyryc.freeservers.com ICQ : 33104110