Hi perhaps I can help you.
If your sefadex is old you can have other properties in your
sefadex and the substance can bind the matrix and the eluted volumen is
often bigger than Vo.
Try to eluted the column one day with your buffer first to use it.
Good Luck
Onyryc wrote:
> How to interpreter these results:
>> Sephadex limits 5000 Daltons
> Vitamin B12 : 1350 Daltons
>> Ve vitamin B12 = 8,7 mL
> Vt sephadex G-25 = 5,3 mL
> Vo = 5,7
>> I don't understand why Ve > Vt, is it possible?
>> --
> - Onyryc -
>onyryc at free.fr>http://onyryc.freeservers.com> ICQ : 33104110
--
Toni Diez Juan
Instituto de Biomedicina de Valencia (CSIC)
Unidad Biologia Vascular
Jaime Roig, 11
46010-Valencia
Spain
Phone: 34-96-339 1751/1750
FAX: 34-96-369 0800
