Hi, there:
I am planning to study HEL-specific B cells in vitro. I know that C57BL/6
mice respond to hen egg lysozyme (HEL) poorly. But someone did set
HEL-specific T cell lines or clones from these mice according to papers (I
can't find more in rencent publications):
a)Manaca F et al. Isolation of lysozyme-specific T cell clones that
discriminate between native and denatured antigen. Cellular Immunology 1994;
154:420-429.
b)Moreno J et al. Co-dominant restriction by a mixed-haplotype I-A molecule
(aKbK) for the lysozyme peptide 52-61 in (H-2k)X(H-2b) F1 mice. J Immunol
1990; 144:3296-3304.
In the second paper given above, I-A transfected L cells were also used in
pair as antigen presenting cells to detect the function of T cells.
If you know who has such kind of T cell clones (T helper or CTL) derived
from C57Bl/6 mice and respond to HEL, or that kind of cells to be used as
APC, please kindly tell me.
Thanks in advance.
Yiqiang Wang
Department of Internal Medicine
EMRB 540
University of Iowa
Iowa city, IA 52242
Tel: 319-335-6842(work)
Fax: 319-335-6887
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