Hi Tina
We routinely use MACS (by Miltenyi Biotech) for T cell isolation by negative
selection and get >95% CD3-positive cells.
What are you sorting and for what purpose? MACS is probably better for
handling large numbers of cells in a relatively short period, but sorting by
cytometer will probably give you better purity. Miltenyi also markets MACS
as a "pre-sort" isolation step prior to sorting by cytometer for small
populations (ex. CD34).
Paula Lavery
Transplant Immunology Lab.
Royal Victoria Hospital
McGill University
Montreal (QC) Canada
"If we knew what we were doing it wouldn't be called research, would it?"
-- Albert Einstein
Tina wrote in message ...
>Which is the best method for cell sorting : MACS versus cytometer cell
>sorting ?
>>Is the isolation of mRNA with MACS good compared with TRIZOL method?
>>Thanks for answers!
>>
