I beleive they are not glycosylated but I do not think that phage-made MAbs
are full-expression antibody. Aren't they just the single chain Fab?
"Mark Haynes" <Mark.Haynes at Mail.TJU.Edu> wrote in message
news:39FF051E.41ABDAA6 at Mail.TJU.Edu...
> I wonder -are phage-made mab libraries are un-glycosylated?
>> Tom Barr wrote:
>> > Hello all,
> > I write requesting advice regarding monoclonal antibody
> > glycosylation. I'm aware that different mAbs have different degrees of
> > glycosylation, and that the same mAbs, produced under varying conditions
> > can display various levels of glycosylation. My question is this, can a
> > mAb be non-glycosylated and is their an assay (preferably simple and
> > quick!) to determine the presence/quantity of carbohydrate moieties on
> > mAbs ? Any advice would be greatly appreciated...
> >
> > Cheers
> >
> > Tom
> >
> > --
> > -----------------------------------------------
> > Tom Barr (mdp96tab at shef.ac.uk)
> > Division of Genomic Medicine (F-Floor)
> > University of Sheffield Medical School
> > Beech Hill Road
> > Sheffield
> > S10 2RX
>
