Dear Bryan,
I think there are 2 solutions to your problem:
1) check the molecular weight of your Dextran: it usually works very well with
high molecular weight dextran (>500.000).
2) you might use a special "Mono-poly resolving medium" you can get from
different companies, which avoids the dextran separation step and gives two
sharp bands of white cells: the upper band is mononuclear cells, the lower one
granulocytes. RBC sediment at the bottom of the tube.
Alberto Clivio
Milano, Italy
