IUBio

IL-2 assay

Kenneth A Frauwirth kfrauwir at dolphin.upenn.edu
Thu Oct 19 13:48:31 EST 2000


On Thu, 19 Oct 2000 17:08:45 +0200, Simone
<sespader at viro.med.uni-erlangen.de> wrote:
>What is the best method to detect IL-2 secretion by CD4+ T-cells???
>Could a capture-ELISA be a sufficient method ???

The two most commonly used methods are ELISA and CTLL response
(CTLL is a cell line dependent on IL-2 for survival and proliferation).

Each has its pluses and minuses, but I think overall they are pretty
similar.

Ken Frauwirth

-- 
Ken Frauwirth  (MiSTie #33025)   kfrauwir at mail.med.upenn.edu
Abramson Cancer Research Institute
University of Pennsylvania
http://mail.med.upenn.edu/~kfrauwir
"Science is not the way to find the answers to all of our questions.
 Science is a way to find better questions." - C. Barrans






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