> I have coated ELISA plates with Ceramide - 1000 - 100 - 10 and 0 ng/ml
(100
> mcl/well) using 2 coating buffres (ph 9.6 and 7.2).
>> Hereafter we added a mouse anti ceramide antibody and a biotinylated
rabbit anti mouse antibody, TMB etc., with no result. All wells gave the
same as the "0" = bagground.
>> Does anybody have experience with coating procedures using ceramide ?
> I know it is a lipide and not a protein.
> Thanks
> Jens R.