You may want to try fMLP (available from Peninsula Labs). The only problem with
this protein is that it activates the neutrophils, so you will get a lot of
other stuff produced as well. The optimal concentration for fMLP is ~0.1um (at
least for neutrophils), but I'm not sure what time frame would be best for
stimulating IL-4 production.
Hope this helps
Bryan
Jens Rainer Hansen wrote:
> I am going to set up an IL4 assay on human lymphocytes (PBMC). Does anybody
> know what to stimulate with (PHA, PMA etc), how long, conc. etc. to get some
> IL4 in the supernatant ?
>> Jens Rainer
