Hi
I am new to the field of Immunology etc, so please excuse me if I ask
something stupid. I want to use an antibody in immunoprecipitation
assays . All I have been provided with are bleeds from rabbit immunized
with peptide from the protein of interest. The peptide has a cysteine
at amino terminus. I have been told that I need to purify that antibody
with that peptide before using it. I have no idea how to go about it.
I would really appreciate any help with this. If you can guide me to a
published protocol (better if you can provide me with one you have used)
and/or any kits I can use. Thanks a lot.
