Pierce sells kits for making columns with your peptide. I used the Sulfo
link kit (cat # 20401/2) which is designed for that cys residue
www.piercenet.com
good luck
Robert
On Tue, 14 Aug 2001 gs207 at yahoo.com wrote:
> Hi
>> I am new to the field of Immunology etc, so please excuse me if I ask
> something stupid. I want to use an antibody in immunoprecipitation
> assays . All I have been provided with are bleeds from rabbit immunized
> with peptide from the protein of interest. The peptide has a cysteine
> at amino terminus. I have been told that I need to purify that antibody
> with that peptide before using it. I have no idea how to go about it.
> I would really appreciate any help with this. If you can guide me to a
> published protocol (better if you can provide me with one you have used)
> and/or any kits I can use. Thanks a lot.
>>>
