This message has been posted by: Derek Davies <derek.davies at REMOVE-THIS-TO-SENDukonline.co.uk>
In article <9kekvl$15io28 at hkunae.hku.hk>, "EddieWK"
<ewkip at hkucc.hku.hk> wrote:
> Anyone have experience in inducing apoptosis on particular human cells,
> like
> Jurkat cells? I would like to know how to efficiently generate high
> percentage of apoptotic cells (early apoptotic, i.e annexin V+ / PI-). I
> did try to use UVB to induce apoptosis of Jurkat cells, but results not
> consistant.
You could try a low concentration of staurosporine (1 uM) or
camptothecin (100 ug/ml) - generally you would be able to pick up
apoptotic changes by about 4-5 hours after induction.
Derek
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