I would like to carry out an adaptation of the apoptosis assessment assay
used by Monti et al. (Mech. Age. Dev. 121: 239-250). Briefly, it would
involve the incubation of whole blood with and without 2-deoxy-D-ribose (as
an oxidative apoptosis inducer) for 48 hours. Apoptosis would then be
measured with Annexin V and PI on the flow cytometer. I cannot find a
protocol on the internet where somebody has carried this out before and so
do not know what quantity of 2-deooxy-D-ribose should be used. Any comments
or advice on this procedure would be most gratefully received.
Regards
Daniel G. Blount
Nottingham University
School of Pahrmaceutical Sciences
University Park
Nottingham
NG7 2RD
UK
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