Hi,
I have a lambda DNA which I have tagged with biotin. Now I want to measure the amount of biotin that has bound to the lambda DNA. I thought about using streptavidin conjugated with alkaline phosphatase and p-nitrophenyl phosphate and measure it spectrophotometrically, but then I have problems with the DNA which has to be immobilized first. Any suggestions?
Sincerely,
Jowita Sleboda
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