hsnel at nizo.nl wrote in <C12569E3.004EC747.00 at mail.nizo.nl>:
>>>>I am looking for equipement for dissociation of lymphoid organs into
>single cell suspension. What company sells this in Holland/Europe? And
>why do most papers refer to stainless steel sieves? Other (i.e.
>disposable) material cannot be used for this purpose?
>>Hans Snel
>>>>---
We gave up using the stainless steel sieves. We now use the disposable
ones ,no more autoclaving, contamination..etc just throw it away and use a
new one.
Becton Dickison/Falcon Sterile 40,70,100 micron mess. Fits right over a
50ml centifuge tube. Break up the spleen/node right into the tube use a
syringe plunger. These are the way to go. There really is no reason not to
use them except "that's the way it was always done"
Brian Hess
