I am trying (without success) to pull down a transiently expressed c-myc epitope tagged protein. From immunocytochemistry labelling, my protein is a soluble nuclear factor. From the precipitation experiments I have done so far, I am getting several background (unwanted proteins) and not mine. It is definitely present in the transfected cells (western blot analysis on total cell lysates). Does anyone have any suggestions. I have tried numerous washing buffers (RIPA, high salt etc) and am fast running o
ut of ideas. Can anyone help?
Thank you
Natalie
nsampson at hgmp.mrc.ac.uk
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