You can culture using RPMI + 20% FCS, although the human serum would be
better. You can culture on plain plastic, although removing the cells
can be a pain
and may require trypsanization.
Hope this helps
Bryan Heit
Immunology Research Group
University of Calgary
Calgary, Canada
Eric wrote:
> Has anyone had any experinec in culutring human
> monocytes? Specifically can I use FCS instead on human AB serum to
> maintain the cells.Also must I culture the cells on special (polyprop
> or Teflon coated) flasks?thanks in advance Eric deszo at uiuc.edu
