I have a problem with immunoprecipitation (ip) in that I get non-specific
binding of recombinant proteins to protein A sepharose while using RIPA
buffer or PBS. I incubate antibody-antigen at 4 degree for 2 h and then add
protein A sepharose to the mixture followed by incubation at 4 degree
overnight while rotating.
Thanks,
Manjili
<http://www.biowww.net/forum/read.php?f=3&i=207&t=207>
