clumps are never good when you are using a column
stemcell technologies inc. may be of some help
they are located in vancouver
you could also isolated mono and then differentiate them to DC
vuvu at freemail.it wrote:
> Hi
> I have problems in immunomagnetic purification of dendritic cells from
> human buffy coats. I'm doing a positive selection of CD14 cells and I
> get variable purity which ultimately affects consistently the quality of
> the cells at later steps. I think that the problem might be at the
> column step when I notice clumps formation. I'd greatly appreciate if
> you could give me your advice.
> Thanks
> Daniela
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