can't you use dapi?
there is also a compound called cyber-green that (ithink) is used like the
taqman probes but they are non-specific binders to dsDNA
call up applied biosystems to search
also a company called molecular probes may help
Amir Hashemi wrote:
> Hi,
>> Is there anybody who knows how to stain ALL MAMMALIAN NUCLEUS by
> indirect immunofluoresence. I need an antibody or a substance which
> interacts specifically with dsDNA or DNA-bining proteins but not with
> RNA. Usually, a compound with such characters is rather cheaper than an
> antibody which explains why the first one is more of our interest .
> However, I have so far not been able to find the right substance since
> firest, the candidate substance should be excitable over 470 nm and
> secondly, we do not want to change our current protocol. For example, to
>> use an anti-H3 from Cell Signaling you have to "unmask the antigen"
> which takes major changes in our current protocol.
>> Sincerely
>> Amir Hashemi
>> ---------------------------------------------------------------------------
>> S. H. (Amir) Hashemi
> Dept of Anatomy and Cell Biology
> University of Göteborg
> Box 420
> SE 405 30 Göteborg
> Sweden
>> Fax +46-31-829690
> ---------------------------------------------------------------------------
