In <3AFFF0F5.70F031BC at Mail.TJU.Edu>, Mark Haynes wrote:
>>can't you use dapi?
>there is also a compound called cyber-green that (ithink) is used like the
>taqman probes but they are non-specific binders to dsDNA
SYBR Green I. Excitation peak at 497 nm and fluorescent emission peak at 520 nm.
Should be viewable with a standard "fluorescein" filter set.
Not stricly specific for dsDNA, but it does have much higher fluorescence when
bound to dsDNA than when it is bound to ssDNA or RNA.
[...]
>also a company called molecular probes may help
Indeed, their handbook is online:
http://www.probes.com/handbook/
Specifically, this section is relevant to the discussion:
http://www.probes.com/handbook/sections/0806.html
Nick
--
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu
