EGFP

[Simon Frost]

Hi,
 I'm looking for memory responses in T cells educated with Dendritic cells
transfected with Flu NP as part of my project. I use DC's transfected with
EGFP expressing  plasmid to monitor transfection efficiency. However, when I
measure T cell activation by ELISPOT the EGFP gives a high background in
some but not all donors. Does anybody know of any characterised epitopes
within EGFP or which HLA backgrounds are responsible.

thanks in advance


frostie