Dear college
We frequently determine CTL activity in immunized mice by interferon
gamma ELISPOT assay. Up to now we have worked with spleen cells. We
assay CTL activity in individual mice. Now, working with some
particular immunogens (e.i, recombinant viruses, via i.p) we have
noted much variability between mice tested of same group. The
variability could be as follow: 453 spots per 10(6) cells, 1786 x10
(6), and 8452 x10 (6), in three mice tested. Working with naked DNA
this have not occurred yet. All this difficult too much any
comparison.
We ask for somebody else with ELISPOT experience:
How many individual mice are recommended to tested in ELISPOT assay?
If you use statistics to compare between groups? What statistic method
do you use?
Any Recommendation or help will be accepted.
Thanks in advance
DiogenesQV
