>>>>> "dq" == diogenes quintana <diogenes.quintana at cigb.edu.cu> writes:
dq> Dear college We frequently determine CTL activity in immunized
dq> mice by interferon gamma ELISPOT assay. Up to now we have
dq> worked with spleen cells. We assay CTL activity in individual
dq> mice. Now, working with some particular immunogens (e.i,
dq> recombinant viruses, via i.p) we have noted much variability
dq> between mice tested of same group. The variability could be as
dq> follow: 453 spots per 10(6) cells, 1786 x10 (6), and 8452 x10
dq> (6), in three mice tested. Working with naked DNA this have
dq> not occurred yet. All this difficult too much any comparison.
dq> We ask for somebody else with ELISPOT experience:
dq> How many individual mice are recommended to tested in ELISPOT
dq> assay? If you use statistics to compare between groups? What
dq> statistic method do you use? Any Recommendation or help will
dq> be accepted.
You might check out the AIDS Vaccine 2001 WWW site (I can't recall
where it is, but google should help). Michael Hudgens was the lead
author on a nice poster regarding data analysis for ELISPOT assays,
from our stat group. This was for HIV vaccines, but should be
applicable.
best,
-tony
--
A.J. Rossini Rsrch. Asst. Prof. of Biostatistics
U. of Washington Biostatistics rossini at u.washington.edu
FHCRC/SCHARP/HIV Vaccine Trials Net rossini at scharp.org
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