Mab affinity

[Geert De Jaeger]

For those having a lot of experience with raising monoclonal antibodies
by hybridoma technology I have the following question:
What is the range of affinities of a panel of monoclonal antibodies
against a certain antigen. Do you  always obtain affinities in the
nanomolar range or does this depend on the antigen and the immunization?
Could someone give me results of affinity determinations of different
panels of antibodies raised against different antigens.

Kind regards,

Geert
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Geert De Jaeger 
DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Vlaams Instituut voor Biotechnologie                           VIB
mailto:gejae at gengenp.rug.ac.be    	   http://www.plantgenetics.rug.ac.be
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