I knew it is unclear that antigen retrieval methods with high temperature
is to break the bonds that were formed during formalin fixation. But
lately I have tried CD3 antigen retrieve Microwave Oven in pH9.0 buffer
(10mM tris.HCl, 1mM EDTA), what is the mechanism? I get good staining,
especially in small intestine, also in inflamed pancreatic islets. But I
may need to see more T-cells in inflamed islets. I need to optimise the
condition, it may help if I knew more the mechanism of the antigen retrieve
in pH 9.0.
Cheng
Cheng Luo, Ph.D.
Medicity Research Laboratory
University of Turku
Tykistökatu 6 A
FIN-20520, Turku
Finland
Tel. +358 2 3337025
Fax +358 2 3337000
Email Cheng.Luo at utu.fi
URL:http://users.utu.fi/cheluo
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