Dear Andy,
Here are some of the possible uses of DID and IEP:
A. DID:
1. Comparing different antigens in a solution having mixture of
antigens.
2. To check specificity of the antisera.
B. SRID:
1. Quantitation of the antigen.
C. IEP:
1. To resolve serum proteins.
2. To check monospecificity of the antisera.
Disadvantage:NEEDS LARGE AMOUNT OF SERA.
YOU CAN SEE THE FOLLOWING REFERENCES:
1.Oudin,J.(1980). Immunochemical analysis by antigen-antibody
precipitation in gels,in Meth.Enzymol.,Vol.70,AP,166-198.
2. Ochterlony,O. and Nilson,L.A.(1986). Immunodiffusion and
Immunelectrophoresis, in Handbook of experimental immunology,Vol 1
(Weir,D.M. et al.),Blackwell,Oxford,pp 32.1-32.5
Good Luck!
Devender Kr. Saini,
Immunodiagnosis Lab,
Dept. Veterinary Parasitology,
College of Veterinary Sciences,
CCS Haryana Agricultural University,Hisar,Haryana,
India-125004.
----- Original Message -----
From: Andy <odonnell975 at btinternet.com>
Date: Sunday, February 17, 2002 4:20 am
Subject: Double Radial Immunodiffusion
> Hi, I was wondering if anyone could tell me of some possible
> practical uses
> for immunoelectrophoresis and double radial immunodiffusion? I
> know it these
> are used to study antibody-antigen interactions, but could anyone
> give a
> possible example? Any help would be greatly appreciated.
>> Andy
>>>
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