I am using an FITC-conjugated secondary antibody on histological
sections of mouse embryos. We get great labeling with our primary
antiserum. However, all the blood cells also label. The blood cells
fluoresce brightly even when no primary antibody is used. I use 10%
serum-0.1% triton to block. There is obviously some non-specific
binding of the secondary Ab-FITC to the blood cells. I tried 4
different secondary Ab conjugated to either FITC or TRITC. HOW CAN I
BLOCK THIS???
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