what species areyou using?
what do you mean all the blood cells stain--in vasculature, red cells,
lymphocytes?
have you done a titration of the secondary Abs?
""Dr. Douglas Darling"" <douglas.darling at louisville.edu> wrote in message
news:3C7CDA5A.445823A3 at louisville.edu...
> I am using an FITC-conjugated secondary antibody on histological
> sections of mouse embryos. We get great labeling with our primary
> antiserum. However, all the blood cells also label. The blood cells
> fluoresce brightly even when no primary antibody is used. I use 10%
> serum-0.1% triton to block. There is obviously some non-specific
> binding of the secondary Ab-FITC to the blood cells. I tried 4
> different secondary Ab conjugated to either FITC or TRITC. HOW CAN I
> BLOCK THIS???
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