"Dr. Douglas Darling" wrote:
>> I am using an FITC-conjugated secondary antibody on histological
> sections of mouse embryos. We get great labeling with our primary
> antiserum. However, all the blood cells also label. The blood cells
> fluoresce brightly even when no primary antibody is used. I use 10%
> serum-0.1% triton to block. There is obviously some non-specific
> binding of the secondary Ab-FITC to the blood cells. I tried 4
> different secondary Ab conjugated to either FITC or TRITC. HOW CAN I
> BLOCK THIS???
Do you use F(ab')2 antibody or whole molecule ?
May be 0.5% BSA would be better as blocking agent ?
