In article <20020701131325.10918.qmail at ww02.hostica.com>,
<tomek_onetpl at poczta.onet.pl> wrote:
>Does anybody know which company sells antibodies specific to human Fab
>(IgM)? Thanks a lot in advance.
You aren't likely to find one. The only mu-specific portion of the Fab
fragment will be CH1, which will be present on intact IgM as well.
The rest of the Fab fragment will be your light chains, which don't
specifically pair with mu, and the variable regions of the heavy chains,
also not unique to IgM.
What you would be looking for is an anti-human mu, CH1-specific antibody,
which may or may not be available. It's likely that at least some anti-mu
monoclonals bind CH1 but the company selling them may not even know which
part of the mu constant region it binds to. Even if it exists, you won't
find it or you'll have to buy a lot of monoclonals and see if the epitope
maps to CH1 specifically.
Perhaps if you described the problem or assay you're working on, you
could get some input on other approaches to whatever you're doing.
Stacy
