why not start with an anti-human antibody for the standard curve?
or if possible use a monoclonal.
I don't know if these reagents are available but I would certainly consider
all possible avenues for the primary antibody + control (in the early stages
at least) you may get a good canine producer and that pups serum may become
your + control.
<paxdgb at nottingham.ac.uk> wrote in message
news:20020717090956.2762.qmail at ww02.hostica.com...
> Hi
>> I am trying to develop an ELISA to detect anti-tetanus antibodies in
canine serum. I have located sources for tetanus toxoid for binding and
anti-canine IgG peroxidase. Does anybody know where I can get a reference
dilution of canine anti-tetanus in order to quantify my values?
>> Alternatviely, does anybody know of a company that either produces a
canine ant-tetanus ELISA kit or maybe would test samples for me (eg. a
veterinary lab)?
>> Any help would be greatly appreciated.
>> Daniel Blount
> PhD Student
>>http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0
