In article <3D08C3DF.1060700 at yale.edu>,
David Johnson <david.johnson at yale.edu> wrote:
>>We typed (our) HeLa as HLA-A*6802, -B*1503 or -B*1537, and -Cw*1604
>HLA-A3, -A28, -Bw15, and Bw35 [Espmark et al Tissue Antigens 11:279-86
>(1978)] and genetically typed as HLA-A68 (a subtype of A28), -B75 (a
>subtype of B15) and NEGATIVE for B35 [Benham, Gromme and Neefjes,
>Obviously, these findings disagree. You might consider HLA class I
Thanks! I think I will try the typing, though at this point my main
question is whether Hela are homozygous at HLA-B, because I want to be
able to say that there are 6 HLA molecules in them. It will become more
important later on.
I'm pretty sure our Hela are not HLA-A3, because they stain negative with
GAP-A3 while transfected HLA-A0302 stains positive in them.
I wonder if the disagreement in typing is because of different methods,
problems with the methods, or because various people have different cells
they call "HeLa" because of cross-contaimination.
Ian
--
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
