Hi,
This is probably a very basic question, but what could cause isotype
cross-reactivity in antibodies? I'm trying to raise rabbit anti-mouse
IgG antibodies by injecting the rabbit with whole mouse IgG (H&L), but
find that the antibodies are also reactive to the other isotypes.
Is this because I'm using whole mouse IgG and the antibody recognizes
the light chains of other antibodies? What should I do instead? Use
only the gamma chain? Is there a way to extract just the IgG
antibodies? Thanks to all who reply!
Dan
