IUBio

TMB substrates

Greg Tobin tobin at bmi-md.com
Tue Dec 2 17:26:18 EST 2003


or less expensive reagents with comparable sensitivity from BMI?
www.bmi-md.com


"Nanci Donacki" <captainnanci at earthlink.net> wrote in message
news:1skmb.1107$X22.591 at newsread2.news.atl.earthlink.net...
> Why not just buy the pre-made 1 component TMB from KPL?
>
> <mclellan12xu at yahoo.com> wrote in message
> news:20031024013346.7709.qmail at ww02.hostica.com...
> > This recipe works very well:
> >
> > 8.2g sodium acetate anhydrous
> > 3.6g citric acid
> > dissolve in 600ml water then add:
> > 270mg TMB (from Fluka) dissolved in 400ml methanol
> > store in dark at 4oC.
> >
> > Add 5 micro litres of 30% hydrogen peroxide per 10ml TMB substrate
> immediately prior to use. Stop with 50microlitres 2N sulphuric acid.
> >
> > Sorry I can't provide an origin or reference for this recipe.
> >
> >
> >
> >
> >
> > http://biowww.net/mynews/tree.php?group_name=bionet_immunology&begin=0
>
>





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