Can anyone tell me if circulating human CD4+ T cells can be directly
(absence of APCs)activated by SEB? Human blood CD4+ cells were sorted
by FACS to >98% puriety. Cells were stimulated for 6 days with SEB (3
micrograms/ml)and sups analyzed by ELISA for IL-13 and IFN-gamma. Very
high cytokines levels were seen (100 ng/ml IFN-g and 5 ng/ml IL-13).
Any thoughts on mechanism? Does SEB bind to a TLR?
Thanks-Becky
