I'm looking for a molecule as a control for flow cytometry. What I need
is a protein with the following characteristics:
o Expressed on many (human) tissue types and cell lines
o Expressed at relatively high levels, so easily detected
o Expressed at relatively constant levels (i.e. minimally sensitive to
cell cycle stage or stress)
o Antibodies easily available
o NOT MHC class I, since that's what I'm trying to control for.
I'm wondering about some of the integrins, perhaps, but I'm not sure which
are widely-enough expressed; but any protein that meets the
characteristics above would be OK.
Ian
--
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
