r94223035 At ntu.edu.tw wrote:
>> I have a guestion about protein concentrator. Would you please do me a
> favor?
>> I concentrate protein in dilute solution by putting it in a dialysis
> bag(Cellu.Sep.H1 MWCO 5000),sprinkle AQUACIDE III over the bag, and
> leave it overnight at 4 degrees. Next morning, I received the clear
> solution and precipitate and both of them were no signals on SDS-PAGE.
> Both of them had UV absorption spectra at wavelenth 280nm. I also
> dissolved Aquacide III which is outside the bag in water and it had UV
> absorption spectra at wavelenth 280nm. Could Aquacide III outside the
> bag pass through the membrane? If it could pass through the membrane,
> what's wrong with my procedures? And how to improve it?
>
I've never used the method you describe but I have had good results using
Centricon type protein concentrators such as the ones listed here...
https://www1.fishersci.com/Coupon?cid=1328&gid=107683&details=Y
They're available for different sample sizes and with different molecular
weight cut-offs.
Eric
