[Immunology] Re: Differentiation of macrophages from PBMC

[bahawi at gmail.com]

Quick answer:

PMA is added to THP-1 to be monocyte like cell, So you do not need to
add PMA for fresh monocyte.
PMA enhances expression of CD14 receptors and may be of TLR4 receptor.
You can use FACS technique to confirm that THP-1 have gained the
monocyte characteristics