We do often do FACS analysis with monoclonal antibodies. As a control
for nonspecific binding of the MAb we use an isotype matched negative
control.
To avoid the problem of nonspecific binding of 2ndary Ab we use
F(ab')2 anti-IgG conjugated to FITC (or other dye). No Fc domain
means no binding to Fc receptors.
HTH
Allison
Sanjay Patel wrote:
> Dear Sir/Madam,
>>>> I'm staining mouse and human whole blood for FACS analysis. Some
> investigators have told me that I need to use Fc block for both, and some
> have said that I need Fc block only for mouse as human whole blood contains
> serum which is a natural block? What should I be using and what is the best
> block for each species?
>>>> Many thanks
>>>>>>>> Sanjay Patel
>
