I am currently investigating the actions of a particular cytokine (a
Wnt family member). My plan was to use conditioned medium from 293T
cells that have been transfected with an over-expression vector and use
the untransfected cells as a control. However, I am not sure whether
primary mouse BM cells will be able to grow in this. What other
factors are secreted by 293T cells, and what additional factors (if
any) would you add?
