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I am trying to setup the FACSCalibur for K562 cells stained with CFSE and 7AAD but I can't get it right. Any help please! Felix Dr. Felix N. Toka DVM, PhD USDA, ARS, Plum Island Animal Disease Center Greenport, L.I., New York 11944-0848 USA Tel.: 631 323 3250 Lab.: 631 323 3264