I am currently working on the human PBMC. The concanavalin A stimulated PBMC
showed high proliferation rate, but the LPS stimulated PBMC had similar
proliferation rate with the unstimulated one. Is different LPS source
stimulate PBMC growth differently? I am using the LPS from Escheriachia
coli, 055:B5, Sigma. What are the possible reasons for low cell
proliferation for LPS stimulated PBMC?
Thank you.
regards,
Tim Tee
Dept. of Pharmacy
Fac of Medicine
University of Malaya
50603 Kuala Lumpur
Malaysia